Thursday, January 5, 2012

Update on flasks and germination times for species and some hybrids

I have been extremely busy with flasks lately. It has been challenging to keep up with all the re-plating while still ordering in seeds from overseas and sowing these and my own stock. Space is a luxury and I hope that by the time I completely run out of incubator space, some flasks will be ready for potting up to free up some space. The Dendrobium species have done well and have grown fast under my conditions. I find that they are not too fussy with media composition but the Bulbophyllum species and Trias species are fussy when it comes to too much residual water in the flasks. Their protocorms prefer to be kept on the dry side, like the Phalaenopsis protocorms and also the Anacheilium cochlaetum. The Rhynchostylis gigantea are shooting roots now and looking healthy. Cynorkis fastigiata is growing all over the place at an incredible rate and benefits well from stronger light it seems. Plants that have not done well are Vanda bensonii and V. denisoniana as well as Mastigeon putidum and Holcoglossum amesianum. The protocorms of the Vandas and M. putidum drowned somewhat in a thin layer of residual water on the surface of the medium but I have managed to save some of these by replating them out early to new drier medium and they appear to be greening up again. The Holcoglossum did well for several months until the second leaf was produced. Even after re-plating they just faded away and I lost these sadly. I am not quite sure what the problem was but I will figure it out eventually.


Bletilla hybrid

Cynorkis fastigiata

Dactylorhisa majalis

Dendrobium draconis

Dendrobium lituiflorum

Dendrobium griffithianum

Dendrobium hercoglossum

Dendrobium heterocarpum

Dendrobium kingianum

Dendrobium findlayanum (Black eye)

Dendrobium parishii

Dendrobium primulinum (yellow)

Disa atricapilla

Disa uniflora

Phalaenopsis hybrid (my own cross)

Polystachya panniculata

Polystachya transvaalensis

Rhynchostylis gigantea

Satyrium coriifolium
Anacheilium cochleatum
For those who are interested to know how long it takes some species and hybrids to germinate at approximately 26 Degrees Celsius with a light regiment of 16hrs:8hrs on/off, I have compiled a list of what I have recorded for the last short while of those I have successfully germinated (in days):

Acrolophia capensis - 33d
Acrolophia cochlearis - 62d
Anacheilium cochleatum - 24d
Angraecum sacciferum - 33d
Angraecum sesquipedale - 22d
Ansellia africana - 13d
Bletilla hybrids - 14-16d
Bletilla striata - 12d
Bonatea speciosa - 30-56d
Bulbophyllum siamense - 25d
Bulbophyllum patens - 30d
Bulbophyllum basicetum - 29d
Cynorkis fastigiata - 18d
Cymbidium aloifolium - 62d
Dactylorhisa majalis - 19d
Dendrobium ravanii - 42d
Dendrobium annosum - 23d
Dendrobium findlayanum - 24d
Dendrobium formosum - 21d
Dendrobium gratiotissimum - 23d
Dendrobium griffithianum - 22d
Dendrobium hercoglossum - 21d
Dendrobium kingianum -12d
Dendrobium lituiflorum - 15d
Dendrobium unicum - 29d
Dendrobium wardianum - 15d
Dendrobium lindleyi - 15d
Dendrobium parishii - 15d
Dendrobium polystrichum - 22d
Dendrobium primulinum - 21d
Dendrobium hercoglossum - 15d
Dendrobium heterocarpum - 15d
Dendrobium sutapense - 15d
Dendrobium palpebrae - 15d
Disa atricapilla - 20d
Disa bracteata - 104d
Disa cornuta - 51d
Disa harveiana - 40d
Disa thodei - 28d
Disa uniflora - 16d
Eulophia angolensis - 42d
Eulophia clavicornis - 50d
Eulophia speciosa - 30d
Eulophia spectabilis - 21d
Gymnadenia conopsea - 26d
Holcoglossum amesianum - 17d
Holothrix incurva - 20d
Microcoelia exilis - 33d
Myrmecophyla tibicinis - 17d
Mystacidium capense - 42d
Phalaenopsis lowii - 21d
Phalaenopsis hybrids - 16d
Polystachya anceps - 28d
Polystachya ottoniana - 15d
Polystachya panniculata - 23d
Polystachya transvaalensis - 30d
Rhynchostylis gigantea - 21d
Satyrium coriifolium - 57d
Satyrium hallackii - 72d
Stenoglottis fimbriata - 14d
Vanda bensonii - 18d
Vanda denisoniana - 18d




8 comments:

  1. Dear
    David V

    Ilike knowing that you culture medium used in vitro germination
    for disa uniflora orchid.
    Regards
    William
    Alajuela, Costa Rica.

    ReplyDelete
    Replies
    1. Hi William. Good to start with half or even quarter strength MS.

      Delete
  2. Dear
    David V

    Ilike knowing that you culture medium used in vitro germination
    for disa uniflora orchid.
    Regards
    William
    Alajuela, Costa Rica. (wb777a@yahoo.es)

    ReplyDelete
  3. HI
    David V
    Thank you for your valuable information, but I have a doubt because I see that there are different formulas or presentations ms which is not all in my country can get from laboratories phytotechnologies.
    Excuse me could help me again.

    ReplyDelete
    Replies
    1. Hi William. If you cannot get media commercially in Costa Rica, you should consider making up the medium from its individual components. You can get product information from Sigma (http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/1/m5519pis.pdf) or if they operate in your area you could get it through them as well as other microprop products they provide. Let me know if this is helpful.

      Delete
  4. Hi David
    M5519= M519
    Thanks very much.

    ReplyDelete
  5. HI
    David
    I forgot to ask if the culture medium M5519 is the same for replate.
    Regards
    William

    ReplyDelete
    Replies
    1. Hi William just make sure you are using M and S and that there is not another medium called M5519. I think the M5519 is just part of the catalogue number from Sigma. You can use the same medium for re-plating but your additives may changed according you your needs.

      Delete